BBa_M45221
BBa_M45221 Version 1 (Component)
post-homologous PpRac-1 section

BBa_M45220
BBa_M45220 Version 1 (Component)
pre-homologous PpRac-1 section

BBa_M31512
BBa_M31512 Version 1 (Component)
section of M13.1 (HpaI to BamHI section)

BBa_M31511
BBa_M31511 Version 1 (Component)
HpaI to BamHI section of M13K07 genome - refactor

BBa_M31337
BBa_M31337 Version 1 (Component)
section of M13.1 from HpaI to BamHI (old)

BBa_M31901
BBa_M31901 Version 1 (Component)
HpaI to BamHI section of M13K07 genome

BBa_M31902
BBa_M31902 Version 1 (Component)
HpaI to BamHI section of M13K07 genome

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.